How cytometer can Save You Time, Stress, and Money.
How cytometer can Save You Time, Stress, and Money.
Blog Article
Details-enriched characterization of cell populations. Morphological information and facts from illustrations or photos can include to your richness of flow cytometry knowledge. By way of example, the determine exhibits an otherwise conventional apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in each populace to expose morphologically distinct attributes. These insights could not have already been received from multiplex staining by itself.
As samples are obtained about the Attune CytPix Flow Cytometer, the higher-pace brightfield digicam captures and stores illustrations or photos of detected functions in a rate as much as 6,000 visuals for each next, dependant upon the flow level and image size. For larger versatility, Attune Cytometric Software enables you to alter the image capture frequency as required and select a specific gate for graphic seize, if needed.
Incorporate acoustic focusing technological innovation with superior-parameter Assessment for versatile spectral unmixing and traditional workflows.
Find new insights into your experimental data by visualizing populations using merged fluorescence and morphology facts. Our computer software interface wizards empower downsampling, concatenation (merged information) and dimensionality applications that happen to be created into the program for an intuitive, streamlined details Investigation practical experience.
*Pricing is indicative only and is matter to vary. Please contact your Agilent consultant with any concerns. The data set forth herein just isn't an offer or a commitment, both of which happen to be subject matter to credit rating approval, terms and conditions, and availability with Agilent. Agilent Monetary Alternatives is offered in selected international locations.
Additionally, it's illuminating to see what number of doublets slip via conventional singlet gates, and terrific to confirm the standing of unanticipated double-positive activities in my Examination."
Optimizing gating system. Even robust handbook singlet gating is error-susceptible and continues to be a subjective selection point in Nearly all flow cytometry assays. Imaging may be used to verify and modify gates to include only solitary cells of desire.
Constant impression quality even at significant throughput Acoustic concentrating as well as a read more high-velocity camera Blend to graphic these T cells constantly at lower or large flow premiums. Effortlessly modify concentrate and camera configurations to satisfy experimental needs.
Early signs of this contamination had in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also lets you use morphological functions to find out interesting subpopulations that might not be clear from flow cytometry knowledge alone.
Probably most of all, these gatherings have cells of Evidently diverse phenotypes which could lead on to incorrect conclusions about double constructive functions (specifically in rare populations).
Sound waves allow specific mobile alignment, causing Outstanding knowledge high-quality and ~10X more quickly sample flow fees.
Application Take note: Automatic graphic Assessment lowers person-to-user variability in flow cytometry gating tactics
Leverage the strength of State-of-the-art technologies and innovation to accelerate your discoveries. Rely on within the Attune Flow Cytometer spouse and children to provide the reliability, speed, and precision that established your function apart.
Adaptable payment program from Agilent Economic Options assist you to get the important instrumentation you require for quick project, or to expand your lab.
Gating on these prolonged parameters means that you can quickly and precisely determine populations of desire and ensure gating tactic with little if any manual evaluate. The Attune computer software features use of state-of-the-art device learning Investigation resources in an expertly made consumer interface, rendering it exceptionally simple to operate.
The Attune Xenith cytometer is actually a large-parameter spectral analyzer, offering both of those spectral unmixing and traditional flow abilities for extensive and functional cell Assessment.
The Attune CytPix Flow Cytometer lets end users to visualise samples all through acquisition, confirm the accuracy of gating approaches, and support benefits with dependable documentation for publication needs.
Listed here, we demonstrate that by gating approaches using graphic-based quantitative parameters can distinguish interacting cells from coincident activities to additional precisely analyze interacting cells.